Project 3 modelling

1. Protein release modelCalculate the fraction of protein released under different conditions.

dc

d50out (cells)Wout (cells)

Product yield after homogenisation

OUTPUT INFORMATIONS

4. Mass Balance for homogenisation

4. Mass Balance for centrifugation

INPUT INFORMATIONS

d50out (debris)Wout (cell debris)

3. Particle size distributionComputing the particle size distribution of slurry enter the centrifuge disc-stack Computing the PSD for sediment streamComputing the PSD for supernatant stream

Homogenisation Modelling

INPUT PARAMETER: Q -- Feed stream flow rate

Centrifugation Modelling

1. Critical DiameterExamine the effect of flow rate on the critical diameter

Product yield after centrifugation

1. Vtotalfeed = 100L2. Total protein concentration = 40g/L3. Product concentration at feed = 107 unit/ml4. Cell concentration at feed = 120g/L5. Cell density = 1100kg/m36. Debris density = 1050 kg/m37. Liquor density = 1000 kg/m3 (assumed)

Protein release R

d50out (in each stream)Wout( in each stream)

INPUT PARAMETER: 1. Pressure 2. Number of passes

2. Particle size distributionExamine the particle size distribution of a slurry stream that containing cells.

3. Cell Disruption PredictionExamine how much the value of the characteristic size (d50) for a given number of passes (N) deviates from its value in the original slurry.

Td

2. Solid RemovalExamine the effect of flow rate on the Grade efficiency (extend of solid removed for supernatant stream)