技术路线
2021-05-16 00:18:47 0 举报
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Chemical Composition and Infrared Sprctroum
Assay of THP-1 cell Proliferation
CCK-8 kit to detect the proliferation of THP-1 cells
Centrifugation ( 4000r 15min)
HFCP Extraction
Sevag method
Infrared Spectroum to detect functional group
Protein
sulfate group
freeze-drying
various Concentration of HFCP
amino sugar
Total sugar
Galacturonic acid
Cell Culture
Drying the treated HFC
95% Ethanol soaked to degrease over night
45% and 75% graded mellow
reducing sugar
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