Chemical Composition and Infrared Sprctroum
Assay of THP-1 cell Proliferation
CCK-8 kit to detect the proliferation of THP-1 cells
Centrifugation ( 4000r 15min)
HFCP Extraction
Sevag method
Infrared Spectroum to detect functional group
Protein
sulfate group
freeze-drying
various Concentration of HFCP
amino sugar
Total sugar
Galacturonic acid
Cell Culture
Drying the treated HFC
95% Ethanol soaked to degrease over night
45% and 75% graded mellow
reducing sugar
