Statistical Analyses
ELISA(IL2/IL4/ IL5/ IL6/ IL10/ IL12/ IL13/ IL17A)
Control
Female wild-type C57BL/6J mice (6-8w)
paraffin embeddingand sectioning
EAE
baricitinib-1 group (3 mL/kg)
Flow Cytometry(Th1/Th2/Th17/Treg)
Western Blot(JAKs/p-JAKs/STATs/p-STATs)
PCR(RORgt/t-bet/GATA-3/Foxp3/GAPDH)
baricitinib-2group (10 mL/kg)
Immunohistochemistry
Inducer