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Membranes were isolated by ultracentrifugation (35000rpm 40min）
impurities were separated by low-speed centrifuge (4000rpm 10min)
Protein was extracted from the membrane in buffer containing 0.5% LMNG for 5–6 hours
Ultracentrifugation to remove unsolubilized material. (35000rpm 40min)
DAY1
protein-containing cell pellets were resuspended in lysis buffer
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