miRNA in early GC

tissue validation

internal validation

Filtering out 2 miRNAs that exhibitedvery low expression in serum and 2 other miRNAs that werehighly correlated with other miRNAs

diagnostic performance of the discovered miRNA candidates

9 miRNAs

logit(P) = (−0.2941 + (0.5042 × miR-18a) + (0.1351 × miR-181b) + (0.1130 × miR-335)).

TCGA

8 miRNAs

logistic regression withelastic net regularization and bootstrapping

multivariate logistic regression and derived model

external validation

biomarker discover

retrospective serum validation

miRNA-based signature for the early detection of GC

3 miRNAs

prospective serum performance evaluation

qRT-PCR

50 pairs

5 miRNAs

10 miRNAs