RNA测序和生物信息学分析全流程图

Gene count TXT format data

aligned BAM format data

Quality check

Data preprocessingon Galaxy platform

Poor samples removal

RNA sequencing

HISAT2: genome alignment

RNA extraction

FastQC: quality inspection

RAW FASTQ data

DEGs screen

Trimmomatic: poor data trim

Final report

RNA integrity

PPI network analysis

Bioinformatic analysis

featureCounts: reads count

Subclusters analysis

RNA concentration

GO terms and KEGG pathway enrichment analysis